Detection protocols for biotinylated probes: optimization using multistep techniques.
نویسندگان
چکیده
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A dot blot hybridization test was developed for the detection of enterotoxigenic E. coli without the use of radioisotopes. Three biotin-labeled DNA (Bio-DNA) probes corresponding to structural genes specifying heat-labile and heat-stable enterotoxins of porcine and human origin were prepared by random priming; label incorporation was significantly higher than that obtained from the use of nick ...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1992
ISSN: 0022-1554,1551-5044
DOI: 10.1177/40.4.1552190